Yolk granule fusion is required for cortical granule translocation towards the oocyte cortex. Related to S5 Fig.
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posted on 2023-06-08, 17:54 authored by Shayan Shamipour, Laura Hofmann, Irene Steccari, Roland Kardos, Carl-Philipp HeisenbergTime-lapse fluorescence movies of exemplary Tg(hsp:clip170-GFP) oocytes labeling the ooplasm (cyan) and exposed to Lysotracker, marking yolk granules (magenta), and DMSO (control), 200 μM Colchicine (Colchi), 100 μM para-Nitroblebbistatin (PBb), or 100 μM Ouabain (30-min interval) during oocyte maturation. Time: 0 to 270 min after maturation induction. Scale bars: 100 μm.
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yet unrecognized rolesubsequent chorion elevationcompetent cortical granulesacentrosomal microtubule astersorchestrating cytoplasmic organizationoocyte actin cortexmaturing zebrafish oocytenet movement towardsmicrotubule aster formationcdk1 upon gvbdyolk granule fusionyolk granuleoocyte surfaceoocyte centercytoplasmic flowsmoved towardscompacting towardsygs fusingvesicular traffickingvesicles decoratedsurface localizationremarkably littleprocess centralpreferentially bindingpositive vesiclesorganelle fusionmaster regulatormain driversfunctioning togetherfindings unravelfinally demonstrateegg activationdynamic changescytoskeletal rearrangementscombined activitiescell polarizationcell migrationcell divisionaccumulate together
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