posted on 2021-12-23, 18:48authored byAlexander J. Weitzel, Hannah A. Grunwald, Ceri Weber, Rimma Levina, Valentino M. Gantz, Stephen M. Hedrick, Ethan Bier, Kimberly L. Cooper
(A-C) Wild-type embryonic ovary. All other panels in each row are Spo11Cas9-P2A-eGFP/+ littermates (e.g., A’–A”‘). (D) Wild-type and (D’)Spo11Cas9-P2A-eGFP/+ adult testis. Scale bar is 50 μm for all panels. Confocal fluorescence imaging settings are identical for all samples; eGFP expression is substantially higher in testes compared to any embryonic ovary time point. IF, immunofluorescence.