posted on 2021-12-23, 18:48authored byAlexander J. Weitzel, Hannah A. Grunwald, Ceri Weber, Rimma Levina, Valentino M. Gantz, Stephen M. Hedrick, Ethan Bier, Kimberly L. Cooper
(A)Spo11Cas9-P2A-eGFP/+ and (B)Spo11Cas9-P2A-eGFP/Cas9-P2A-eGFP seminiferous tubules; scale bar is 50 μm. Cells in seminiferous tubules in (B) likely do not complete meiosis, evidenced by absence of sperm. (C) Tiled image of Spo11Cas9-P2A-eGFP/Cas9-P2A-eGFP testis; scale bar is 200 μm. All seminiferous tubules are deformed and devoid of sperm.