Detection of total dsRNA using dot-blot.

Parchure, Adwait; Cesarec, Mia; Braut, Antonija; Kolman, Robert; Ivanišević, Vlatka; Čunko, Marina; Bursać, Slađana; de Reuver, Richard; Begonja, Antonija J.; Rosani, Umberto; Volarević, Siniša; Maelfait, Jonathan; Jurak, Igor

doi:10.1371/journal.ppat.1012452.s007

Detection of total dsRNA using dot-blot.

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posted on 2025-04-08, 23:42 authored by Adwait Parchure, Mia Cesarec, Antonija Braut, Robert Kolman, Vlatka Ivanišević, Marina Čunko, Slađana Bursać, Richard de Reuver, Antonija J. Begonja, Umberto Rosani, Siniša Volarević, Jonathan Maelfait, Igor Jurak

WT and ADAR1 KO were infected with HSV-1 (MOI=3). At 7h.p.i., cells were collected either in TRIreagent and total RNA was extracted. a) Total RNA blotted on charged membrane with positive and negative control. (LEFT) Image captured with HRP linked secondary. (RIGHT) Colorimetric image. b) ImageJ quantification of dot blot (n=18). Data is shown as mean ± standard deviation (SD); ‘ns’ not statistically significant, by One-Way ANOVA for (b)

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Detection of total dsRNA using dot-blot.

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