Rate of incorporation of radiolabeled leucine and uracil into purine-starved and purine-replete cells.
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posted on 2014-02-27, 02:58 authored by Jessica L. Martin, Phillip A. Yates, Radika Soysa, Joshua F. Alfaro, Feng Yang, Kristin E. Burnum-Johnson, Vladislav A. Petyuk, Karl K. Weitz, David G. Camp II, Richard D. Smith, Phillip A. Wilmarth, Larry L. David, Gowthaman Ramasamy, Peter J. Myler, Nicola S. CarterThe rate of incorporation of [4,5-3H]-leucine and [2-14C]-uracil was compared between purine-replete (open bars) and cells starved for purine for 24 h (closed bars). Rates were calculated based upon 3 biological replicates per time-point for each condition (purine-replete versus purine-starved) and the data represent the mean rate of incorporation from two independent assays.
