Polarity of Nuclear Actin Filaments Reflect the Overall Polarity of the Cell
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posted on 2013-02-22, 08:34 authored by Becket Feierbach, Silvia Piccinotti, Margaret Bisher, Winfried Denk, Lynn W EnquistNeurons were stained with AF568-phalloidin, anti-GM130 to stain the Golgi. GFP-VP26 is visualized by direct fluorescence. Each image is a 2-D projection from four consecutive layers in a confocal image stack, taken 0.5 μm apart. Scale bar = 20 μm. Top two rows show polarized SCG neurons with one axon. Bottom row shows a single SCG neuron with two axons emanating from opposite sides of the cell body.
