Overexpression of ALS3 in the bcr1/bcr1 Mutant Restores Substantial Biofilm Formation In Vitro

Biofilms were grown under standard conditions and stained with concanavalin A conjugate for CSLM visualization. The top panels show the visual appearance. The next set of panels show depth views, in which blue color represents cells closest to the silicone and red color represents cells farthest from the silicone. The next set of panels show side views. For the depth views of reference strain DAY185 (BCR1/BCR1), blue = 0 μm and red = 600 μm; CJN1149 (BCR1/BCR1 + TEF1-ALS3), blue = 0 μm and red = 500 μm; CJN702 (bcr1/bcr1), blue = 0 μm and red = 80 μm; CJN1153 (bcr1/bcr1 + TEF1-ALS3), blue = 0 μm and red = 180 μm. For the side views, the scale bars represent 50 μm for DAY185 (BCR1/BCR1), CJN1149 (BCR1/BCR1 + TEF1-ALS3), and CJN1153 (bcr1/bcr1 + TEF1-ALS3); and 20 μm for CJN702 (bcr1/bcr1). The next set of panels show RT-PCR analysis of ALS3 expression of the indicated strains with successive 2-fold dilutions of cDNA from left to right. The bottom panels show RT-PCR of control TEF1 transcript levels.