Microtubule nucleation after releasing from nocodazole treatment.

At 48 h post transfection with either non-targeting (panel A-J) or YB-1 siRNA (panel K-T), HeLa cells were infected with influenza virus at MOI of 10. At 8 h post infection, cells were treated for 1 h without (Noc(-)) or with 1 μg/ml of nocodazole (Noc(+)). At 3 (panel C, H, M, and R), 5 (panel D, I, N, and S), and 15 min (panel E, J, O, and T) post releasing from nocodazole treatment, cells were fixed and subjected to indirect immunofluorescence assays with mouse anti-α-tubulin antibody. Arrowheads indicate the microtubules sporadically localized at the peripheral cytoplasm (panel R and S). Scale bar, 5 m.