Lipidomics of T. gondii tachyzoites identifies a novel parasite lipid, PtdThr.
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posted on 2015-11-13, 02:54 authored by Ruben D. Arroyo-Olarte, Jos F. Brouwers, Arunakar Kuchipudi, J. Bernd Helms, Aindrila Biswas, Ildiko R. Dunay, Richard Lucius, Nishith Gupta(A) Elution profile showing the retention times and relative abundance of lipids isolated from extracellular tachyzoites (107). X1 represents a previously unknown lipid. (B) MS analysis of X1 fraction revealing PtdThr, PtdSer, and PEtn-Cer species. Individual lipids were identified by their fragmentation patterns and m/z ratios in the negative ionization mode. (C) MS/MS spectrum of X1-derived major peak (m/z 850.5) from panel B. Note the neutral loss of 101 Da (transition from 850.5 to 749.6). Acyl chains (sn-1, 20:1; sn-2, 20:4) were identified by their masses.
