Blocking GABAergic transmission in the honeybee changes amplitude but not duration of the KC population response.

(A) Temporal response profile of the calcium signal imaged in the mushroom body lip region of one honeybee for different stimulus durations as indicated by color. (B) Temporal response profiles as in (A) in one honeybee after application of PTX. (C) Response profiles imaged from 6 control animals (gray) and their average (black) for a 3 s stimulus as indicated by the stimulus bar. The responses measured in 6 animals in which GABA_A transmission was blocked with PTX (red) shows a considerably higher population response amplitude. The shaded area indicated the standard deviation of responses across bees. (D) Average amplitude of responses that are normalized per animal are highly similar in animals treated with PTX and control animals. (E) Blocking GABA_B transmission with CGP in 6 animals (blue) again results in an increased response amplitude compared to 6 control animals (black). The shaded area indicates the standard deviation across individuals. (F) Average normalized (per animal) response profiles are highly similar in the CGP-treated and control animals.