ZEB1 and ZEB2 expression and survival analysis in AML.

(A) Violin plots depicting normalized gene counts as TPM of ZEB1 (left) and ZEB2 (right) gene expression across AML samples aggregated by the presence of genomic gene fusions present in human AML [23]. Median including 75% and 25% quartiles are denoted from top to bottom as dashed lines. (B) Kaplan–Meier analysis of samples containing high (blue line) or low (red line) expression of ZEB1 (left) and ZEB2 (right). The appropriate cutoff was defined through a scanning method implemented in the R2 database (http://r2.amc.nl). Bonferroni corrected p-values determine that higher ZEB1 statistically reduce the overall survival probability (S9B Fig, left), while higher expression of ZEB2 did not cause this effect. *p < 0.05; **p < 0.01, ***p < 0.001, Bonferroni-corrected nonparametric t test. (C) Increased expression of both ZEB1 (left) and ZEB2 (Right) appear to significantly correlate with increased numbers of leukemic blasts present in AML populations (p = 0.0417 and p = 0.0024, respectively). Raw data behind plots are included in S7 Table. AML, acute myeloid leukemia; TPM, transcripts per million.

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