Two-photon imaging of mouse parabrachial nucleus neurons in acute brain slice.

(A) ROI segmentation of SomaFRCaMPi-expressing neurons in the parabrachial nucleus. Left: Representative image of single FOV (top) expressing FRCaMPi, blue square is magnified in the bottom panel. Right: Corresponding ROI masks extracted using Cellpose. (B) Similar to (A), but in SomaFRCaMPi-expressing neurons. (C) ROI number extracted per FOV for SomaFRCaMPi and FRCaMPi experiments (SomaFRCaMPI; 2 mice, 5 brain slices, 22 FOVs. FRCaMPi; 2 mice, 4 brain slices, 18 FOVs). (D) Example heatmaps for 15 min of activity from two example brain slices (4 simultaneously acquired FOVs per slice). (E) Example traces demonstrating calcium waveform from (D). The quantitative data presented in this figure can be found in S2 Data.

(EPS)