Trehalose promotes intracellular lipid accumulation in Aag2 cells.

(A) Intracellular lipids were labeled in Aag2 cells maintained in either glucose (GLU) or trehalose (TRE) cell culture media using either vehicle control (DMSO) or Nile Red. Cells were fixed and digital images were obtained at 20X magnification. Hoescht nuclear stain was added to each condition. (B) The corrected total cell fluorescence (CTCF) was quantified using the integrated density of 60 individual cells per condition. Assays were performed in triplicate. Normality and unpaired Student’s t tests were performed between groups to assess statistical significance. Standard deviations are shown.