Thermal shift experiments reveal hysteresis of the temperature-responsive bistable switch.
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posted on 2016-12-22, 18:34 authored by Aaron Mischa Nuss, Franziska Schuster, Louisa Roselius, Johannes Klein, René Bücker, Katharina Herbst, Ann Kathrin Heroven, Fabio Pisano, Christoph Wittmann, Richard Münch, Johannes Müller, Dieter Jahn, Petra DerschY. pseudotuberculosis expressing ProvA-egfpLVA was grown to a continuous culture in a chemostat at 25°C, shifted for 8 h to 37°C and back to 25°C for 18 h. Bacteria were analyzed by (A) flow cytometry (mean ± SEM; n = 3 for each temperature; 105 cells per replicate), or (B) by western blot. Relative RovA amounts were quantified using ImageJ (mean ± SEM; n = 3 for each temperature; c: a protein band unspecifically recognized by the antiserum served as loading control).
