The subcellular localization of OASL-V5 and ORF20-myc isoforms is unaltered in latent and reactivated HuARLT2-rKSHV.219 cells.

HuARLT2-rKSHV.219 cells were transiently transduced with lentiviruses encoding the indicated construct. 4 days post transduction, cells were seeded onto glass coverslips. The following day, cells were untreated (A, latent infection) or reactivated with sodium butyrate and RTA-expressing baculovirus (B, reactivated). Infected cells express GFP, reactivated cells express RFP, and transduced proteins were detected with anti-myc or anti-V5 antibodies and an anti-mouse Alexa Fluor 647 coupled antibody (pink). Nuclei were counterstained with Hoechst (blue). Images are representative of at least 2 independent experiments. Scale bar = 10 μm.