The autoinhibited structure of V. angustum LuxO-RC is physiologically relevant.

Bioluminescence produced by a V. cholerae reporter strain carrying LuxO-controlled luciferase. Light production values for mutant strains are normalized to that produced by inactive, wild-type LuxO (WT). Mutations introduced into V. cholerae LuxO with the goal of disrupting the R-C interface display reduced bioluminescence—signifying increased LuxO activity—comparable to the constitutively active phosphomimetic LuxO D61E. The underlying data can be found in S1 Data.