Fig 3.TIF (1.34 MB)
The autoinhibited structure of V. angustum LuxO-RC is physiologically relevant.
figure
posted on 2016-05-24, 07:40 authored by Hande Boyaci, Tayyab Shah, Amanda Hurley, Bashkim Kokona, Zhijie Li, Christian Ventocilla, Philip D. Jeffrey, Martin F. Semmelhack, Robert Fairman, Bonnie L. Bassler, Frederick M. HughsonBioluminescence produced by a V. cholerae reporter strain carrying LuxO-controlled luciferase. Light production values for mutant strains are normalized to that produced by inactive, wild-type LuxO (WT). Mutations introduced into V. cholerae LuxO with the goal of disrupting the R-C interface display reduced bioluminescence—signifying increased LuxO activity—comparable to the constitutively active phosphomimetic LuxO D61E. The underlying data can be found in S1 Data.
History
Usage metrics
Categories
Keywords
Vibrio choleraevirulence factor productionATPase superfamilyLuxO activationAutoinducer binding inactivatesautoinducers triggers repressionreceiver domainsLuxO oligomerbiofilm formationhistidine sensor kinase familyresponse regulator LuxOvibrio autoinducer molecules bindtransmembrane receptorsrecapitulates elementsAAAchemical signal moleculescrystal structureautoinhibited stateinhibitor moleculereceiver domain phosphorylation
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC