Subcellular co-localization assay of mCherry-NbATG8f with YFP or p20-YFP in Nicotiana benthamiana leaves.
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posted on 2025-02-24, 18:59 authored by Yongle Zhang, Zuokun Yang, Zhe Zhang, Guoping Wang, Xiang-Dong Li, Ni HongThe infiltrated leaves were treated with 100 µM E-64d at 48 h post-inoculation and examined at 12 h post E-64d treatment. Scale bars, 20 µm. The co-localization was further analyzed by overlapping fluorescence spectra, and areas indicated with white dashed lines in enlarged sections were used for this analysis.
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molecular mechanism underlyingviral factors sequestercitrus tristeza virussgs3 ternary complexdiv >< pgene silencing 3related protein 8decreased viral accumulationviruses exploit autophagyshowed milder symptomsnicotiana benthamiana maim5 maim1 antiviral rna silencingsgs3 related protein atg8attenuated ctv infectionvirus accumulationbenthamiana related genesrna silencingnbgapcs nbatg7 nbatg5 binding proteinsgs3 degradationsgs3 ),sequestered sgs3specific rnap20 proteinviral suppressortwo atg8taken togethersuccessful infectionregulated genesproviral roleopting autophagymutated ctvinvestigation foundinteracting motifsinduce autophagyctv infectionautophagy negativeautophagy inductionautophagic substratesautophagic degradation
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