Stage-dependent biomarker levels.

Lethal factor levels in animals with fast and slow progression, and survivor, at the end-of-phase-1 (A) and 48 hours (B). Box plot is for median and quartiles, error bars for minimum and maximums, excluding outliers, median levels and p-values included for Wilcoxon rank sum test. (C) LF, EF levels (upper panel), and LF/EF ratio (lower panel), during earliest detection in phase-1 (P1), at the end-of-phase-1 (EOP1), subsequent phase-2 (P2), and phase-3 (P3) for fast progression (left panel) and slow progression (right panel). Lines connect the medians and error bars give quartiles for each stage. Red dashed line represents the LF threshold and blue line the EF threshold for phase-2. P-values included for testing differences in toxin levels and toxin ratios between phases. Black font includes all parameters unless another color font is shown for a specific parameter. Black rectangle shows similarity of low LF/EF ratios at the EOP1 for fast progression and P3 for slow progression.