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posted on 2021-10-12, 17:23 authored by Erin L. Attrill, Rory Claydon, Urszula Łapińska, Mario Recker, Sean Meaden, Aidan T. Brown, Edze R. Westra, Sarah V. Harding, Stefano Pagliara

(A) Temporal dependence of bacterial population size when phages were continuously injected in the mother machine device at an MOI of 103 (triangles) or 105 (squares). Data and error bars are the average and standard error of the mean of 530 (triangles) and 208 (squares) single-compartment measurements from biological triplicate experiments in the mother machine. The dashed lines are guides for the eye. Some of the error bars are hidden behind the corresponding data points. (B) Corresponding temporal dependence of the concentration of phages collected in the mother machine output. Data are the mean and standard error of the mean of biological triplicates. Dashed lines are guides for the eye. Some of the error bars are hidden behind the corresponding data points. Dotted lines indicate the constant supply of phages in the mother machine input for the 2 MOIs. Data above each respective dotted line indicate phage amplification; data below the corresponding dotted line indicate the absence of phage amplification due to lysis inhibition. Numerical data for each replica are provided in Data J and Data O in S1 File. MOI, multiplicity of infection.

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