Root-mean-square deviation (RMSD) of Cα positions, a measure of distance between corresponding atoms in two structures, for the different domains of FVa in the the membrane-containing simulations (Memb #1-#6) based on various superimposed selections.

The different panels include RMSD calculation for multiple superimposed selections each corresponding to a specific superimposed alignment applied before calculating the RMSD, enabling comparison between both the different simulations and domains of FVa. The term “si” indicates which selection was superimposed before RMSD calculation.

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