PLOD1 shRNA depletion and small molecule inhibition with 2-DP destabilize EBNA1 protein.

A) Raji cells transduced with lentivirus shControl (shCtrl) or shPLOD1 (pool a) were assayed by Western blot for PLOD1, EBNA1, EBNA2, LMP1, or Actin at 7 days post-transduction. Each lane is a biological replicate. B) Raji (left) or LCL (right) treated with DMSO or 2,’2-dipyridil (2-DP, 200 μM) were assayed by Western blot for PLOD1, EBNA1, EBNA2, LMP1, or Actin. C) Raji (left) or LCL (right) were treated with DMSO, 2-DP (200 μM), 2-DP+MG132 (10 μM), or 17-DMGA (1 μM) for 48 hrs and assayed by Western blot for EBNA1 (top) or Actin (bottom). D) Raji (left) or LCL (right) were treated with DMSO, 2-DP (200 μM), or CoCl₂ (100 μM) for 48 hrs followed by Western blot for PLOD1, EBNA1, HIF1α, or Actin.