Overview of Siphoviridae genera Dhillonvirus, Nonagvirus, and Seuratvirus.

(A) Schematic illustration of host recognition by small siphoviruses. (B) Representative TEM micrograph of phage TheodorHerzl (Bas14). (C) Color code of terminal receptor specificity (same as in Figs 3 and 4). (D) Maximum-Likelihood phylogeny of the Dhillonvirus genus based on a whole-genome alignment with bootstrap support of branches shown if >70/100. Newly isolated phages of the BASEL collection are highlighted by orange phage icons and the determined or proposed terminal receptor specificity is highlighted at the phage names using the color code highlighted in (C). The phylogeny was rooted between phage WFI and all others based on a representative phylogeny including Drexlerviridae sequences as outgroup (S1A Fig). (E) Maximum-Likelihood phylogeny of the Nonagvirus and Seuratvirus genera based on a whole-genome alignment with bootstrap support of branches shown if >70/100. Newly isolated phages of the BASEL collection are highlighted by orange phage icons, and the determined or proposed terminal receptor specificity is highlighted at the phage names using the color code highlighted in (C) (see S2B Fig for the 3 phages without coloring). The phylogeny was rooted between the 2 genera that belong both to the Queuovirinae subfamily of Siphoviridae. (F) The results of quantitative phenotyping experiments with Dhillonvirus, Nonagvirus, and Seuratvirus phages regarding sensitivity to altered surface glycans and bacterial immunity systems are presented as EOP. Data points and error bars represent average and standard deviation of at least 3 independent experiments. Raw data and calculations are available in S1 Data. Abi, abortive infection; BASEL, BActeriophage SElection for your Laboratory; EOP, efficiency of plating; RBP, receptor-binding protein; RM, restriction–modification; TEM, transmission electron microscopy.