NbNRC2 elutes as a dimer in analytical gel filtration assays.

Gel filtration assays with NbNRC2. C-terminally 6xHis-3xFLAG tagged NbNRC2 was expressed and purified from leaves of nrc2/3/4 KO N. benthamiana. Purified protein was run on an S200 10/300 analytical column, and the estimated molecular weight of the NRC2 peak was calculated based on comparison of the elution volume to a protein standard curve ran on the same column. Elution volume (Ve) and molecular weight (Mw) in kDa are shown above the peak. A range of fractions below the peak, marked in green, were ran on SDS-PAGE and visualised using Coomassie Brilliant Blue staining (green panel). Approximate molecular weights (kDa) of the proteins are shown on the left of the green panel. The data underlying this figure can be found in S6 Data.

(EPS)