Mutation of the E-L-E-F-N motif is sufficient for Eph receptor detargeting.

A Dose-dependent inhibition of KSHV infection by soluble EphA2-Fc on SLK cells. KSHV wt or gH-ELAAN were pre-incubated with EphA2-Fc. Fc alone and PBS were used as controls. GFP expression as indicator of infection was measured by flow cytometry. Infection without protein (PBS control) was set to 1 (duplicates, error bars represent range). B Dose-dependent inhibition of RRV infection by soluble EphB3-Fc on SLK cells. RRV 26–95 wt, ΔgL or gH-AELAAN were pre-incubated with EphB3-Fc. Fc alone and PBS were used as controls. YFP expression as indicator of infection was measured by flow cytometry. Infection with Fc 0.8nM was set to 1 (duplicates, error bars represent range). C Target cells were pre-incubated with a soluble ephrinA4-Fc fusion protein at 2μg/ml for 30min prior to infection with KSHV wt or gH-ELAAN. Infection was measured as in A. Infection without protein (PBS control) was set to 100% (triplicates, error bars represent sd). Non-normalized infection (%GFP+ cells) ±sd is listed below the respective bars. D Target cells were pre-incubated with a soluble ephrin-Fc fusion protein mix (ephrinA1, ephrinA2, ephrinA3, ephrinA4, ephrinA5, ephrinB1, ephrinB2, ephrinB3) at 2μg/ml each for 30min prior to infection with RRV 26–95 wt, ΔgL or gH-AELAAN. Infection was measured as in B. Infection without protein (PBS control) was set to 100% (triplicates, error bars represent sd). Non-normalized infection (%YFP+ cells) ±sd is listed below the respective bars. ns: not significant, *: p-value < 0.05, **: p-value < 0.01, ***: p-value < 0.001.