MYMV AC4 suppression of transgene-induced PTGS.
(A) Accumulation of green fluorescence in leaves (top and middle panels) and plants (bottom panel) of N. benthamiana 16c infiltrated with 35S-GFP alone or in combination with 35S-AC4 or 35S-P19. Constructs and time points are shown at the top and left side of the images, respectively. (B) GFP mRNA and siRNA extracted from infiltrated (LL) and systemic (SL) leaves of the plants illustrated above. Constructs and time points are shown at the top and bottom of the images, respectively. M, mock-agroinfiltrated plants. rRNA represents ethidium bromide-stained ribosomal RNA shown as loading control.