ppat.1007481.g005.tif (1.23 MB)
M117 interacts with E2F transcription factors and with itself.
figure
posted on 2018-12-10, 18:52 authored by Eléonore Ostermann, Stefan Loroch, Zhikang Qian, Albert Sickmann, Lüder Wiebusch, Wolfram Brune(A, B) NIH-3T3 cells were infected with WT MCMV or MCMVs expressing 3xFlag-tagged full-length (FL) M117 or UL117 or mutant M117 proteins (MOI of 3 TCID50/cell for 24 hours). Cell lysates were subjected to immunoprecipitation (IP) using an anti-Flag antibody. Co-precipitating proteins were detected by Western blot analysis. (C) NIH-3T3 cells were transfected with pcDNA3 expression plasmids encoding the same 3xFlag-tagged proteins as above. (D) NIH-3T3 cells were co-transfected with pcDNA3 plasmids expressing HA-tagged M117 and 3xFlag-tagged FL or mutant M117. Anti-Flag IP and Western blot analysis were done as above.
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E 2F activationnovel E 2F activatorRPEM 117 mediates E 2F interactionprecluding MCMV replicationM 117E 2F transcription factor familyE 2F activatorhost range determinantC-terminal part mediates self-interactionadenovirus E 4orfCMV host restrictionDNAnovel host range determinantMCMV M 117MCMV replicationE 2F transcriptionhost range CytomegalovirusesE 2F target gene expressionmouse cytomegalovirus M 117 proteinM 117 protein
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