Localization of intracellular reporter proteins.
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posted on 2018-02-16, 18:42 authored by Dennapa Saeloh, Varomyalin Tipmanee, Kin Ki Jim, Marien P. Dekker, Wilbert Bitter, Supayang P. Voravuthikunchai, Michaela Wenzel, Leendert W. HamoenB. subtilis strain HM771 (dnaN-gfp, reporter for impaired replication), strain UG-10 (recA-gfp, reporter for DNA damage), strain 1048 (rpoC-gfp, reporter for impaired RNA synthesis), and strain 1049 (rpsB-gfp, reporter for impaired protein synthesis) were grown until an OD600 of 0.3 and subsequently treated with 1x MIC of rhodomyrtone. Scale bar 2 μm.
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ATP synthaseacylphloroglucinol rhodomyrtoneintracellular proteinelectron microscopymembrane-inserting moleculerhodomyrtone transientlyrhodomyrtone causesAberrant protein localization impairsantibiotic candidatemembrane proteinsform intracellular vesiclesmembrane curvaturebinding modeintracellular targetsmyrtle Rhodomyrtus tomentosacauses distortionform protein-trapping membrane vesiclesmembrane invaginationsPreceding studiesamphipathic structurenovel mechanismmembrane fluidizationnovel antibiotic rhodomyrtone traps membrane proteinsdynamics simulationsphospholipid head groups
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