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Immunofluorescence and live-cell microscopy of Pf332-expressing parasites.
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posted on 2023-03-31, 18:04 authored by Yvette S. Levray, Bianca Bana, Sarah J. Tarr, Emilia J. McLaughlin, Peter Rossi-Smith, Anita Waltho, Georgina H. Charlton, Riccardo Zenezini Chiozzi, Colin R. Straton, Konstantinos Thalassinos, Andrew R. Osborne(A-D) Immunofluorescence labelling of parasites expressing the indicated mCherry tagged proteins. Intrinsic mCherry fluorescence of the proteins is shown in red. Labelling with anti-MAHRP1 or anti-plasmepsin V is shown in green. (E) Immunofluorescence labelling of parasites expressing ER-lumenal GFP1-10 only. Parasites were labelled with anti-GFP (red) and anti-plasmepsin V (green). (F) Phase contrast and fluorescence images of parasites expressing the Pf332:Int-mCherry:C-S11:DSLE proteins either alone or with the indicated GFP1-10 proteins. Scale bar: 2 μm.
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div >< pparasite endoplasmic reticulumhydrophobic sequence enterparasitophorous vacuole membranecleaved hydrophobic segmentparasite plasma membranecompletely block exportlike hydrophobic sequencescanonical eukaryotic solublemalaria parasites exportparasite secretory pathwaylumenal topology withininternal hydrophobic sequenceproteins containing transmembraneexported membrane proteinsproductive export intermediatesecretory pathwaymembrane proteinstransmembrane segmenttransmembrane proteinsparasite ertrafficked withinproductive intermediatemalaria infectioner membranelike proteinsexported proteinsexport processexported transmembranelumenal statelumenal specieslumenal intermediateslumenal domainretention sequencetypical solubletransport acrosstrafficked viasignal peptideproteolytically removedprotein exportplasmodium novel meansmodel systemskdel receptorintact cellsexported acrossblood stage
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