Identification of RSV vsRNAs putatively targeting primary miR-263a.

(A) Relative RNA levels of the top ten most abundant vsRNAs in nonviruliferous (N) and viruliferous (V) fourth-instar planthoppers (n = 8). NS, no significant difference. **, P<0.01. ***, P<0.001. (B) Identification of vsR-1524 and vsR-3397 in N and V planthoppers by northern blot using biotin-labeled LNA oligonucleotide probes. (C) and (D) Relative RNA levels of vsR-1524, vsR-3397, and miR-263a in six organs of viruliferous adult planthoppers (C) and in the planthoppers at different days post-injection with RSV crude extractions (D) (n = 8). Different letters indicate significant differences in Tukey’s multiple comparison test. Planthopper U6 snRNA was used as an endogenous control for vsRNA in each experiment. Data information: Graphs show mean values and standard errors.