High-sensitivity checkerboard analysis using Bliss independence model.

(A) Antibiotic checkerboard data with linear concentrations ranges for E. coli, P. aeruginosa, and S. aureus tested against the antibiotics AMP, CIP, GEN, and CTX. Each antibiotic was tested with 8 equally spaced linearly increasing concentrations (denoted 1–8) between zero and the MIC for the respective species. Data are average growth values of 3 biological replicates relative to untreated controls. (B) Dose-dependent synergy profiles according to a Bliss independence additive model. Numerical values for this figure are available in S1 Data.

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