HSF2 binds to the BZLF1 promoter and remodels the chromatin locally.

(A) Schematics of the EBV-BZLF1 promoter region, transcription start site (TSS and black arrow) and HSF2 binding site (yellow box) are shown. The regions amplified in ChIP-qPCR (blue segments) and their nucleotide positions are labeled. Below the HSE consensus logo and the corresponding nucleotide sequence within the BZLF1 promoter are shown. (B) ChIP-qPCR analysis of endogenous HSF2 binding to the BZLF1 promoter regions expressed as % of input in AGS BX1 cells. (C) HSF2 or GFP control overexpressing AGS BX1 cells were used to immunoprecipitated H3K27-me3 followed by RTqPCR for the indicated primers targeting different regions of the BZLF1 promoter. The bars are colored according to the schematic in (H). Bars represent the average and error bars the SD across three independent experiments, data points are indicated as grey circles.