External dsRNA was transported in plant tissues.

AFolRDR1-dsRNAs and GFP-dsRNA were sprayed on the 2-week tomato seedling leaves. The different tissues of treated plant including leaf, stem and root were collected at different time points for detecting dsRNA by RT-PCR. B Fluorescein-labeled dsRNAs were daubed on one side leaf followed by checking the fluoresce signals using CMLS after 24 hours. Scale bars, 1 mm. C The fluoresce signals on undaubed leaf were detected using CMLS 3 days after treatment described above. Scale bars, 1 mm. D The fluoresce signals in stem were detected using CMLS 3 days after treatment described above. Vascular bundles were pointed by white arrows. Scale bars, 1 mm. E The fluoresce signals in root were detected using CMLS 3 days after treatment described above. Vascular bundles were pointed by white arrows. Scale bars, 1 mm.