Expression and analysis of HCMV glycoprotein B (gB).
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posted on 2021-08-05, 17:52 authored by Dipanwita Mitra, Mohammad H. Hasan, John T. Bates, Michael A. Bierdeman, Dallas R. Ederer, Rinkuben C. Parmar, Lauren A. Fassero, Quntao Liang, Hong Qiu, Vaibhav Tiwari, Fuming Zhang, Robert J. Linhardt, Joshua S. Sharp, Lianchun Wang, Ritesh TandonHCMV gB was expressed and purified for GAG binding assays as described in Materials and Methods. Protein purification was verified by SDS-PAGE followed by staining with Coomassie Brilliant Blue. (A) Coomassie protein gel of purified CMV gB as well as flow thorough and washes. (B) CMV gB protein gel lane analysis was performed by using Bio-Rad Image Lab Software. Coomassie purity of gB was determined to be 100% and molecular weight was 107.59 kD. Lane 1—protein ladder (Bio-Rad Precision Plus Protein Dual Color Standards), Lane 2—CMV gB column flow through, Lane 3—1st wash, Lane 4—2nd wash, Lane 5—blank, Lane 6—CMV gB.
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