Exchangeable apolipoproteins can compensate for the role of Erns in the infectious particle formation of pestivirus.
(A) Schematics of the wild type, Erns deletion (CSFVΔErns), and polymerase dead (GAA) CSFV RNA, and the experimental procedure. (B) Expression of the full length of HA-tagged Erns (HA-Erns), HA-ApoA1, HA-ApoC1, and HA-ApoE was determined by immunoblotting at 48-h post-transduction of lentiviruses into SK6 cells. Intracellular CSFV RNA (C) and extracellular infectious titers (D) were determined at 72-h post-electroporation with CSFVΔErns by qRT-PCR and focus-forming assay, respectively. (E) Expression of HA-Erns, Hel, and HA-ApoE was determined by immunoblotting at 48-h post-transduction of lentiviruses into SK6 cells. Intracellular CSFV RNA (F) and extracellular infectious titers (G) were determined at 72-h post-electroporation with CSFVΔErns by qRT-PCR and focus-forming assay, respectively. In all cases, asterisks indicate significant differences (* p < 0.01) versus the results of the control cells.