Distribution of excitatory and inhibitory neuron markers in the superficial and deep layers of the zebrafish OB.

All images represent planes located 110 μm below the ventral surface of the OB. (A) Confocal microscopy image of the OB of a Tg(vglut2a:DsRed) adult zebrafish labeling olfactory glomeruli and mitral cell somata. (B) Confocal microscopy image of the OB of a Tg(gad1b:DsRed) adult zebrafish labeling GABAergic interneurons. (C) Raw fluorescence two-photon image of an OB injected with Rhod-2 AM in the deep layers. The inner dashed line indicates where the interneurons were detected (Fig 5). The outer dashed line indicates the outline of the OB. Scale bars represent 50 μm. Orientation is the same in all pictures as in (A). DsRed, red fluorescent protein; gad1b, glutamate decarboxylase 1; OB, olfactory bulb; Rhod-2 AM, acetoxymethyl ester of rhodamine-2; vglut2a, vesicular glutamate transporter 2.

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