Cryo-EM structures of the FMDV-AWH-W125 complex and FMDV-AWH-W2 complex.

The central cross-sections through cryo-EM maps of the FMDV-AWH-W125 complex (A) and FMDV-AWH-W2 complex (D) are shown with icosahedral 2-, 3- and 5-fold axes. Each image in the 480-pixel boxes corresponds to 446 Å in each dimension. Scale bars, 100 Å. Rendered images of the FMDV-AWH-W125 complex (B) and FMDV-AWH-W2 complex (E). Depth cueing with color is used to indicate the radius (< 120 Å, blue; 130–150 Å, from cyan to yellow; > 160 Å, red). The icosahedral five- and threefold axes are represented by pentagons and triangles, respectively. Footprints of W125 (C) and W2 (F) on the FMDV surface. Each figure shows a 2D projection of the FMDV surface produced using RIVEM [43]. The 5- and 3-fold icosahedral symmetry axes are marked as pentagons and triangles, respectively, on one icosahedral asymmetrical unit. The spherical polar angles (θ, ϕ) define the location on the icosahedral surface. The depictions are radially depth cued from blue (radius = 130 Å) to red (radius = 155 Å). The W125 and W2 footprints are pink-colored.