Cryo-EM images and Fourier shell correlation curves.

(A) Two bands for phage particles separated through CsCl density gradient centrifugation. (B) Cryo-EM images of P22 particles from the upper (left) and lower (right) bands. (C) Structural resolutions of the overall symmetry-mismatch of the mature virion and procapsid as well as the dimer, trimer, tetramer, and C-loop of the SP in the procapsid were estimated based on Fourier shell correlation (FSC) criteria by using the gold-standard procedure. (D) Structural resolutions of the portal in the P22 procapsid and portal–tail complex in the P22 mature virion. The cryo-EM density maps (EMD-61452, EMD-61453, EMD-61454, EMD-61455, EMD-61456, EMD-61457, EMD-61460, EMD-61461) have been deposited in the EM Data Bank.

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