Connectivity map (CMAP) pathway and network analysis for LY-294002 target genes.

Gene list generated to obtain the drug profiles for LY-294002 (S5 Table) was utilized to compile the CMAP “LY-294002 target-gene” list, and genes prospectively up-regulated are presented as a heatmap in (A) showing enrichment in later oligodendrocyte (OL) lineage cells and down-regulation in dorsal neural stem cells (NSCs) or transient amplifying progenitors (TAPs). aEPs, adult ependymas; aNPs, adult neuronal precursors (NPs); GM, grey matter; astros, astrocytes; imOLs, immature OLs; mOLs, mature/myelinating OLs. (B) Prospectively up-regulated or down-regulated genes analyzed by Genego Metacore for GO Pathway Maps and Process Networks, and lists are ranked according to significance (false discovery rate [FDR] <2%)/numbers of genes present in each of the categories. (C, D) Short path network to visualize highly connected signaling-to-transcriptional nodes were performed for up- and down-regulated by LY-294002 target genes. Internal clusters were grouped by selecting the “link GO objects” in GeneGo Metacore. Highlighted blue objects are directly within data and the remaining are within the background (or basal) data.