Comparative affinities of A and G istotype of Fab 43 and 177 to clades A, and C gp140 and to clade B gp41.

Recombinant clades A (A), and C (C) gp140 and clade B gp41 (B) were each immobilized on a CM-5 chip for surface plasmon resonance evaluation of antibody affinity constant. Fab 43A, 43G, 177 A or 177G at the indicated concentrations were the analytes. The KD and corresponding Pearson’s Chi2 test (Chi2) values shown were estimated by global curve fitting of the specific binding responses. Fitted curves are in different colors corresponding to increasing analyte concentration, as indicated below each graph. Injections were carried out in duplicates and gave essentially the same results. Only one of the triplicates is shown.

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