Cellular-level anisotropy models and data.

(A) Integrated areal and directional conflict model side view from 4 DAI canvas start shape to resultant canvas shape at 10.5 DAI, coloured for cell anisotropy. Lines show orientation of the cell long axis and are shown where anisotropy exceeds 0.23. Grey region shows approximate location of mouth. (B) Experimental data showing traps with cells segmented and coloured for cell anisotropy at time points corresponding to those shown for the integrated model shown in (A), side view. Grey region shows approximate location of mouth where visible. (C–D) Experimental data; arrows highlight region of cell anisotropy parallel to the ventral midline. (C) Trap with cells segmented and coloured for cell anisotropy, front view. (D) Additional segmented data. (E–G) Zoomed-in resultant model front views. (E) Areal conflict model; arrow highlights region of cell anisotropy perpendicular to the ventral midline. (F) Directional conflict model; arrow highlights region of cell anisotropy parallel to the ventral midline. (G) Integrated model; arrow highlights wider region of cell anisotropy parallel to the ventral midline. Magenta line shows ventral midline; red line shows dorsal midline. Grey region shows mouth. Colour scale shows cell anisotropy. In all images, cell-shape anisotropy is defined by R − 1/R + 1, where R is the ratio of the long/short axis of an ellipsoid fitted to the cell. This equation evaluates to 0 for isometric cell shape and 0.333 when the long axis is twice the short axis. Data https://doi.org/10.6084/m9.figshare.8966153.v1, Figs 9, 10, S4 and S6.7z archive. Models: http://cmpdartsvr3.cmp.uea.ac.uk/wiki/BanghamLab/index.php/Software and https://doi.org/10.6084/m9.figshare.8966153.v1, Models.7z archive. DAI, days after initiation.