CEP90 depletion affects BB’s distal end maturation.
figure
posted on 2022-09-07, 17:33 authored by Pierrick Le Borgne, Logan Greibill, Marine Hélène Laporte, Michel Lemullois, Khaled Bouhouche, Mebarek Temagoult, Olivier Rosnet, Maeva Le Guennec, Laurent Lignières, Guillaume Chevreux, France Koll, Virginie Hamel, Paul Guichard, Anne-Marie Tassin(A) Paramecium showing 1 ciliated BB after control RNAi depletion (Ctrl). The TZ is characterized by 3 plates indicated by colored arrowheads: terminal plate (green), intermediate plate (blue), and axosomal plate (magenta). (B-E) Upon CEP90 depletion, undocked BB are detected close to the cell surface (C, E) or deep in the cytoplasm (B, D). Vestigial structures resembling the axosomal plate (magenta arrowhead) and intermediate plate (blue arrowhead) are detected on all internal BB (n = 63). (E) Example of rare BB displaying microtubule extensions on the free, uncapped side of the BB (white arrow). Scale bar = 200 nm. BB, basal body; RNAi, RNA interference; TZ, transition zone.
History
Usage metrics
Categories
Keywords
numerous genes involvedfunctional analysis performedstudy basal bodybasal body maturationdiv >< ptransition zone assemblydistal appendage assemblymammalian cells demonstratecep90 functional moduleassembly basal body dockingbasal bodymammalian cellscilia assemblybasal bodiestransition fibersparamecium distal enddistal appendageswidely usedsevere ciliopathiesrecruited earlyplasma membranemolecular playersmodel systemmnr ),future positionfunctionally conservedfully knownexternal surfaceevolutionary conservationcilium biogenesiscellular processcell surface
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC