ppat.1008473.s004.tif (1.78 MB)

Additional insight into the potential of ORF20 to interact with other RHIM-containing proteins.

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posted on 10.07.2020 by Megan Steain, Max O. D. G. Baker, Chi L. L. Pham, Nirukshan Shanmugam, Yann Gambin, Emma Sierecki, Brian P. McSharry, Selmir Avdic, Barry Slobedman, Margaret Sunde, Allison Abendroth

Representative confocal fluorescence spectroscopy time profiles collected from (A) ORF201-114-mCherry and ORF201-114mut-mCherry with YPet-RIPK1497-583, and (B) YPet-ZBP1170-355mutA or YPet-ZBP1170-355mutB fusion proteins with ORF201-114-mCherry. Proteins were incubated alone or mixed in pairs under conditions that allow co-assembly. The proteins present in each mixture are indicated for each part of the figure. Inserts show detail of 1 s of the dual fluorescence recordings, at the time indicated by a star on the full time trace.