Activated vMCs during fear memory test.

(A) Experimental design. Left: After the virus was fully expressed, mice were taken off Dox for 2.5 days and subjected to CFC training in context A, following by a diet with Dox. Three days later, mice were placed in context A for fear memory test and perfused 1.5 h after for immunostaining. (B) Representative image of Calb2⁺ (red) and cFOS⁺ (green) immunostaining in the vDG. Nuclei were stained with DAPI (blue). Scale bar, 100 μm. (C) The proportion of vMCs (Calb2⁺) to all cells (DAPI⁺). Similar percentage of vMCs among all cells in the vDG hilus between the WS and SS groups. (D) The proportion of activated vMCs (Calb2⁺ cFOS⁺) to all cells (DAPI⁺). Similar percentage of activated vMCs among all cells between the WS and SS groups. (E) The proportion of activated vMCs (Calb2⁺ cFOS⁺) to all vMCs (Calb2⁺). Similar percentage of activated vMCs among vMCs in the vDG hilus between the WS and SS groups. In S5C–S5E Fig, statistical comparisons were performed using unpaired t tests. Data were presented as mean ± SEM. The underlying data and statistical information in S5 Fig can be found in S1 Data. The mice depicted were created with BioRender.com.

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