A six-gene cluster plays a role in spore development.

(A) Schematic of the location of the six genes in the spore-related cluster and position of hygromycin cassette integration. (B) Plots of log₂ (counts per million) over spherulation from data in Fig 1 for each gene in the cluster. DIT1/DIT2/DTR1 names from S. cerevisiae orthologs and “Cutinase” name based on Pfam hit to that domain. (C) Traces demonstrating chromosomal location of fold enrichment of ChIP signal/input in spherules (yellow) and hyphae (blue) at the designated time points relative to D8B26_005436 – D8B26_005438 genes. (D) Representative TEM images of wild type and each DitCluster∆ mutant. The scale is the same for all images. (E) Quantification of arthroconidia cell wall width measurements of TEM images for wild-type and each DitCluster∆ mutant. ***: p < 0.0001, by unpaired t test. Underlying data can be found in S16 Table.