Poor conservation can lead to loss of edge connection.

(A) Gene isoforms due to AS events. Constitutive exons are shown in blue and alternatively spliced regions in yellow. Introns are represented by solid lines, and dashed lines indicate splicing options. (B) Two gene isoforms and their sequenced reads. The red lines represent reads sequenced from exons shared by both isoforms. The purple and green lines represent reads that are exclusively sequenced from each isoform. Both isoforms can be aligned with Family X. The isoform containing exons 1, 2, and 3 has a global alignment while the isoform containing exons 1 and 3 produces two local alignments.