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Identification of the 5′ UTR-binding proteins.

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posted on 2009-11-06, 02:08 authored by Yohei Watanabe, Naohiro Ohtaki, Yohei Hayashi, Kazuyoshi Ikuta, Keizo Tomonaga

(A) Silver staining of sequential RNA column purified proteins is shown. The bands specific for the X/P UTR probe (X/P) are indicated. M/G represents a control RNA column using the 5′ UTR of M/G mRNA. (B) The specificity of identified proteins was determined by Western blotting using antibodies specific to each protein (see Materials and Methods).

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