Effects of IFNγ treatment on NAD(P)H inside chlamydial inclusions.

HEp-2 cells were infected with C. trachomatis for 24 hours and treated with 10 units /ml IFNγ 24 hours prior to and during the infection (right panels) or were left untreated (left panels). (A) Color-coded images of τ2-NAD(P)H (scale bar = 20 µm). Arrows point to persistent chlamydial inclusions harbouring enlarged reticulate bodies. (B) Color-coded images of the relative amount of protein-bound a2-NAD(P)H (scale bar = 20 µm). Arrows point to persistent chlamydial inclusions harbouring enlarged reticulate bodies. (C) IFNγ induced persistence decreases τ2-NAD(P)H inside the chlamydial inclusion. Quantitative analysis of τ2-NAD(P)H (n = 27 ROIs from three independent experiments; mean ±SEM) at 24 hpi. (D) IFNγ induced persistence decreases the sizes of chlamydial inclusions. Quantitative analyzes of chlamydial inclusion sizes at 24 hpi (n = 27 inclusions from three independent experiments; mean ±SEM). (E) IFNγ induced persistence increases the ratio of a1/a2 inside the chlamydial inclusions. Quantitative analysis of ratio of free to protein-bound NAD(P)H (a1/a2) (n = 27 ROIs from three independent experiments; mean ±SEM).