Transcriptome analysis of <i>lincRNA-IBIN</i> overexpressing flies reveals changes in metabolic genes that result in increased glucose levels in the hemolymph.

<p><b>A)</b><i>lincRNA-IBIN</i><sup><i>7</i></sup> overexpression with the <i>C564-GAL4</i> driver increases <i>lincRNA-IBIN</i> expression in uninfected flies by 161-fold. This induction is not visible upon a <i>E</i>. <i>cloacae</i> or <i>M</i>. <i>luteus</i> infection, since the infection induces strong <i>lincRNA-IBIN</i> expression. <b>B)</b> Cluster analysis of genes up-and downregulated in <i>C564>lincRNA-IBIN</i> flies compared to <i>w; lincRNA-IBIN</i> controls. The major cluster of induced genes consists of glycoside hydrolase enzymes, whereas enzymes involved in proteolysis form the main downregulated cluster. Clustering was done using GO Biological Process and InterPro protein classification (GO BP/InterPro). <b>C,D)</b> Expression differences of selected <i>Drosophila</i> genes involved in metabolism with and without <i>lincRNA-IBIN</i> overexpression and with and without an infection based on the transcriptome analysis. <b>E)</b> <i>lincRNA-IBIN</i> is induced in the <i>Drosophila</i> adult midgut by a <i>E</i>. <i>cloacae</i> septic injury 24h post infection. <b>F)</b> Workflow of the collection of hemolymph for glucose and trehalose measurements. <b>G,H)</b> Circulating hemolymph glucose levels are elevated upon <b>G)</b> infection and <b>H)</b> overexpression of <i>lincRNA-IBIN</i> in the adult midgut.</p>