Transcriptome analysis of lincRNA-IBIN overexpressing flies reveals changes in metabolic genes that result in increased glucose levels in the hemolymph.

A)lincRNA-IBIN⁷ overexpression with the C564-GAL4 driver increases lincRNA-IBIN expression in uninfected flies by 161-fold. This induction is not visible upon a E. cloacae or M. luteus infection, since the infection induces strong lincRNA-IBIN expression. B) Cluster analysis of genes up-and downregulated in C564>lincRNA-IBIN flies compared to w; lincRNA-IBIN controls. The major cluster of induced genes consists of glycoside hydrolase enzymes, whereas enzymes involved in proteolysis form the main downregulated cluster. Clustering was done using GO Biological Process and InterPro protein classification (GO BP/InterPro). C,D) Expression differences of selected Drosophila genes involved in metabolism with and without lincRNA-IBIN overexpression and with and without an infection based on the transcriptome analysis. E) lincRNA-IBIN is induced in the Drosophila adult midgut by a E. cloacae septic injury 24h post infection. F) Workflow of the collection of hemolymph for glucose and trehalose measurements. G,H) Circulating hemolymph glucose levels are elevated upon G) infection and H) overexpression of lincRNA-IBIN in the adult midgut.