Residue 97 of A3H partially determines the sensitivity to SIVcpz Vifs.

(a, b) SIVcpzPttMB897 or SIVcpzPtsTAN1 wild type or delta vif reporter viruses were produced in 293T cells in the presence of A3H wild type or mutant expression plasmids, as indicated. The viral infectivity was determined by measuring the nanoluciferase of 293T cells infected with normalized amounts of SIVcpz reporter viruses. (c, d) cpzA3H, hA3H wild type or mutants expression plasmids were co-transfected with SIVcpz (SIVcpzPttMB897 or SIVcpzPtsTAN1) Vif expression plasmids into 293T cells. The presence of A3H and Vif was detected by using anti-HA and anti-Vif antibodies, respectively. Tubulin served as a loading control.