More proinflammatory cytokine and chemokine secretion after acute administration of CCl₄.

(A) Representative views of HE, TUNEL, and DAPI staining in the liver sections of med23^f/f and med23^Δli mice after acute CCl₄ administration for 24 hours. (B) Quantification of TUNEL-positive cells from the liver sections of med23^f/f and med23^Δli mice (med23^f/f, n = 6; med23^Δli, n = 5). (C) The total protein was extracted from whole livers of med23^f/f and med23^Δli mice and analyzed by western blotting using the indicated antibodies. GAPDH was used as a loading control. (D, E) Representative views of Ki67 staining (D) and quantification (E) in the liver sections of med23^f/f and med23^Δli mice (med23^f/f, n = 5; med23^Δli, n = 4). (F) qRT-PCR analysis of c-Myc in whole-liver extracts of med23^f/f and med23^Δli mice (med23^f/f, n = 6; med23^Δli, n = 5). (G, H) qRT-PCR analysis of cytokines and chemokines in whole-liver extracts of med23^f/f and med23^Δli mice (med23^f/f, n = 6; med23^Δli, n = 5). Data are presented as means ± SEM. *P < 0.05, **P < 0.01. For underlying data, see S1 Data file. γ-H2AX, phosphorylation of the histone variant H2AX; Ccl, C-C motif chemokine ligand; CCl₄, carbon tetrachloride; Cxcl, C-X-C motif chemokine ligand; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HE, hematoxylin–eosin; Il, interleukin; med23, Mediator complex subunit 23; med23^Δli, liver-specific knockout of Med23; med23^f/f, med23-floxed; qRT-PCR, quantitative real-time PCR; Tnfα, tumor necrosis factor alpha.